The slice was dewaxed at 50?C for 1?h, chromized and washed; the nucleus was stained with Regauds hematoxylin for 5?min; and the section was washed again, stained with Ponceau S acid fuchsin for 5?min, bathed in 2% glacial acetic acid, differentiated using a 1% phosphomolybdic acid aqueous remedy for 5?min, […]